製品説明
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測定対象
アレルゲン > その他ナッツ類、 -
サプライヤ
R-Biopharm - 測定原理
PCR法 - 保管温度
常温 - 毒劇物情報
なし
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (S3301) containing 40 mg hazelnut / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.
The method is validated for quantitative determination of 1 mg to 400 mg allergenic substance / kg food sample using the reference material SureFood® QUANTARD Allergen 40 containing 40 mg allergenic substance / kg food sample.